Search Results for "Stem Cell"

Adult Stem Cells and Regeneration Part 6 of 6

3 Sep 2008 ... Click here to see original video.This lecture by Dr. Nadia Rosenthal discusses the importance of adult stem cells in the tissue maintenance, ... www.e-biotek.com/bio-video.html?task=videodirectlink&id=209

Bioprotocols--mouse stem cell isolation

This website is all about biotechniques. It includes hundreds of databases, bioprotocols, biomedical movies, ebooks, buffer formats, pathways, and education ... www.e-biotek.com/protocols/ebiotek_012.htm

Review Articles on Embryonic Stem Cell Research - p9

15 Jan 2010 ... The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic stem cell ... www.e-biotek.com/.../261-review-articles-on-embryonic-stem-cell-research-.html?...

Bio Video

Dr Andrew Corbett presents the Stem Cell and Human Cloning Debate from an ethical ... What's the difference between embyonic and adult stem cells? ... www.e-biotek.com/bio-video.html

BioProtocols--stem cell research techniques and protocols

Stem cell research has an enormous potential to transform scientific understanding and ultimately improve the quality of human life. ... www.e-biotek.com/protocols/ebiotek_319.htm

Bio Video

The C\'elle menstrual stem cell comes from the uterine lining (endometrium) ... These menstrual stem cells are unique because they have many properties and ... www.e-biotek.com/bio-video.html?task=allvideoslist&from=90

BioProtocols--C1

Feeder-independent culture of human embryonic stem cells - Nature . ... Although the derivation of new human embryonic stem cell lines in those defined . ... www.e-biotek.com/protocols/ebiotek_019.htm

The Science of Transgenic Technology Part 1 of 2

3 Sep 2008 ... This lecture by Dr. Nadia Rosenthal discusses the importance of adult stem cells in the tissue maint... Adult Stem Cell. ... www.e-biotek.com/bio-video.html?task=videodirectlink&id=196

www.DNATUBE.com Rnai interference video

20 Aug 2008 ... Stem Cells and ... Dr Andrew Corbett presents the Stem Cell and Human Cloning Debate from an ethical perspective... What's the diff. ... www.e-biotek.com/bio-video.html?task=videodirectlink&id=178

Re:First Human Embryonic Stem Cell Lines Approved for - Biology Forum

11 Nov 2010 ... This site provides biomedical protocols and databases in life science. In addition, it hosts a bio-forum.. Re:First Human Embryonic Stem ... www.e-biotek.com/bio-forum.html?func=view&id=59&catid=15 Everything You Wanted To Know About Stem Cells... But... 2 Aug 2008 ... Click here to see original video.Google Tech Talks July 31, 2007 ABSTRACT Stem cell technology and the debate surrounding it has generated a ... www.e-biotek.com/bio-video.html?task=videodirectlink&id=30 What's The Difference Between Mitosis and Meiosis? 15 Aug 2008 ... Stem Cells and ... Dr Andrew Corbett presents the Stem Cell and Human Cloning Debate from an ethical perspective... What's the diff. ... www.e-biotek.com/bio-video.html?task=videodirectlink&id=66 Glycolysis Overview Animation for Cellular Respiration 2 Aug 2008 ... Stem Cells and ... Dr Andrew Corbett presents the Stem Cell and Human Cloning Debate from an ethical perspective... What's the diff. ... www.e-biotek.com/bio-video.html?task=videodirectlink&id=19 BioProtocols--video,animation 3 Simple cell by Terry Brown. Southern Blot Animation Dolan DNA Learning Center. Stem Cell Biology and... Stem Cell Research Dolan DNA Learning Center ... www.e-biotek.com/protocols/ebiotek_202.htm Bio Video Stem Cell Biology and Human Diseases. This celebratory scientific symposium marks the 50th anniversary of the description of the structure of DNA by James ... www.e-biotek.com/bio-video.html?task=uservideoslist&from=80...62 Immunology and Histology - Biology Forum Cell Assay Methods ... Stem Cell Methods ... Specific Cell Isolation and Culture ... FCM ... Apoptosis Assays ... Transfection and Transformation . ... www.e-biotek.com/index.php?Itemid=74&option=com...func... Biotechniques--DNA,RNA,Protein,PCR,Animal Experiments,Immunology ... Establishment and characterization of human embryonic stem cell line. More: FCM: Sodium and Potassium Analysis · FCM ：PARP PE · Membrane Potential Analysis ... www.e-biotek.com/ Cell Transfection Protocols 10 Jul 2008 ... Here are some protocols for cell transfection. ... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols ... www.e-biotek.com/cell/97-cell-transfection-protocols.html BioProtocols--endothelial cell 1 Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial .... Stem cell & related protocols · Apoptosis and related protocols ... www.e-biotek.com/protocols/ebiotek_259.htm BioProtocols--cell cycle 2 Preparing Cells for FACS/PI Analysis (Cell Cycle Analysis). www.flemingtonlab.com. .... Stem cell & related protocols · Apoptosis and related protocols ... www.e-biotek.com/protocols/ebiotek_203.htm XTT Cell Viability Assay Protocol 7 Jul 2008 ... Here are some protocols for XTT Cell Viability Assay. ... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols ... www.e-biotek.com/cell/62-xtt-cell-viability-assay-protocol.html General cell culture protocols Cell cycle assay · Migration assay · Stem cell & related protocols · Apoptosis and related protocols · Soft Agar Assay for Colony Formation ... www.e-biotek.com/protocols/ebiotek_044.htm Mitochondrial DNA Isolation 6 Jul 2008 ... A minimum of 2-3 g wet weight of cells are necessary for one DNA extraction ... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and ... www.e-biotek.com/dna/53-mitochondrial-dna-isolation.html RNA MicroArray Protocol 7 Jul 2008 ... Transfer lysed cell solution to a 15 ml flacon tube. ... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols ... www.e-biotek.com/rna/63-rna-microarray-protocol.html Deoxyribose Isolation from DNA Degrasion 6 Jul 2008... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols · Laser Capture Microdissection Protocols · Cell Transfection ... www.e-biotek.com/dna/54-deoxyribose-isolation-from-dna-degrasion.html BioProtocols--Cell11 Determination of Cell Colony Formation in a High Content Screening . ... Stem cell & related protocols · Apoptosis and related protocols ... www.e-biotek.com/protocols/ebiotek_121.htm Comet Assay The Single Cell Gel Electrophoresis assay (also known as comet assay) is an ... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols ... www.e-biotek.com/genetics/99-comet-assay.html SELEX—in vitro selection ... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols · Laser Capture Microdissection Protocols · Cell Transfection Protocols ... www.e-biotek.com/rna/104-selexin-vitro-selection.html Competitive ELISA Protocols 7 Jul 2008... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols · Laser Capture Microdissection Protocols · Cell Transfection ... www.e-biotek.com/immunology/92-competitive-elisa-protocols.html Genomic DNA Isolation from Specific Samples 6 Jul 2008... Radioimmunoassay--RIA Protocols · Stem Cell Techniques and Protocols · Laser Capture Microdissection Protocols · Cell Transfection ... www.e-biotek.com/.../52-genomic-dna-isolation-from-specific-samples.html

养生保健极品--胚胎干细胞

Yes, We Can!

美国科学家发现，人体干细胞的分裂代数是有限的，当从受精卵开始的万能干细胞到多潜能胚胎干细胞，再到成体干细胞共分裂50代（相当于120岁左右)就不再分裂，生命即将终止。即使是没有器质性疾病的百岁寿星或得道高僧，活到120岁就可能会无疾而终，或涅槃西去。所以，对中老年

人来说，定期输注一定数量的胚胎干细胞，就是在生命的银行账户里存上一笔款。此外，人到中年后，可能会有一些疾病，胚胎干细胞具有自我更新和多能转化的特性，能修复器官的损伤，提高免疫力，平衡内分泌，延缓衰老；输注干细胞，就象是在平安保险公司里，为您的健康再买一份保险。

给我一天，还你百年！

人类早已告别了“人到七十古来稀”的时代。按干细胞理论推测，现代人类的平均寿命可以达到100-120岁。打个通俗的比喻，老天爷平均给我们36500元，只要我们每天花费0.9-1元，就正好能活到100-120岁。那么，当代人类平均寿命为什么没有达到100-120岁呢？环境污染、战争、瘟疫、烟酒、不良饮食、精神紧张、…使得我们每人每天的平均支出远大于1元，使得我们的平均自然寿命打了个7-8折，甚至是6折。

补充胚胎干细胞，就是在您的生命银行里在存上3650元，抑或7300元，延长您的寿命；补充胚胎干细胞，修复损伤的器官，提高免疫力，平衡内分泌，清除自由基，使得您每天的支出减为0.8元，抑或0.7元，使您的自然寿命不被打折，还您100-120岁的自然寿辰。

杭州蓝翔生物干细胞特点：

美国技术，中国制造；

纯度高、质量好，能自我更新和多能转化；

安全、有效，无毒副作用；

静脉输注，使用方便；

提高免疫力、平衡内分泌、清除自由基；

抗衰老、抗肿瘤、抗亚健康，防治更年期综合症；

保养卵巢，增加纯天然雌激素的产生--保健、减肥、美容、呈年轻态；

保养睾丸和肾脏，提高性能力；

辅助治疗心血管病、脑血管病、糖尿病、慢性肺病、肝病等疾病，促进手术后的康复。

胚胎干细胞技术--被美国时代杂志评为二十世纪十大科技之首！

1998年美国有两个小组分别培养出了人的多能( pluripotent )干细胞，为人类干细胞研究开创了新纪元： James A. Thomson在 Wisconsin大学领导的研究小组从人胚胎组织中培养出了干细胞株。他们使用的方法是：人卵体外受精后，将胚胎培育到囊胚阶段，提取 inner cell mass细胞，建立细胞株。John D. Gearhart在 Johns Hopkins大学领导的另一个研究小组也从人胚胎组织中建立了干细胞株。他们的方法是：从受精后5－9周人工流产的胚胎中提取生殖母细胞( primordial germ cell )。由此培养的细胞株，证实具有全能干细胞的特征。

人的衰老，就是细胞的衰老；人的疾病，就是细胞的疾病。养生保健和抗衰老，首选杭州蓝翔生物的胚胎胚胎骨髓间充质干细胞。防病治病，可以使用胚胎干细胞。

干细胞技术被美国时代杂志评为二十世纪十大创新科技之首。干细胞，是生命的源泉—人体由干细胞发育而来；干细胞是生命的动力—它贯穿于生命过程的始终，更新衰老、死亡的细胞，修复病损细胞；干细胞是青春永驻的新希望—它能使全身器官年轻化，延缓衰老；干细胞是再造组织器官的种子--它可以分化为各种功能细胞，用于生产人体组织和器官；干细胞是生命的“银行”—它犹如一座尚待开发的“金山”，取之不尽用之不竭；干细胞是疾病的克星—它可用于治疗多数人类疾病，特别有用于疑难杂症。

胚胎干细胞--重塑您的生命抛物线；

胚胎干细胞--为您的人生轨迹导航；

胚胎干细胞--为您的健康保驾护航。

———————————————————————————————————————

杭州蓝翔生物科技有限公司：杭州市凤凰城1幢1单元2703室，电话571-86069948, 传真571-86069948, 邮箱： beststemcell@hotmail.com

网址：www.beststemcell.com

www.beststemcell.com--杭州蓝翔生物科技有限公司

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Stem Cell Therapy Gaining Ground in Asia

Heda Bayron | Hong Kong 25 November 2010

Asians are beginning to warm up to the idea of using their own stem cells to treat a host of illnesses such as heart disease, cystic fibrosis or leukemia. Some parents are preserving their babies' umbilical cords, hoping that as technology advances their children can use the umbilical blood to cure future illnesses.

It is a nightmare for parents to hear their child has developed a disease that requires a bone marrow transplant. For instance, patients with leukemia, a type of blood cancer, sometimes have a hard time finding a bone marrow match.

Scientists say the umbilical cord that attaches a baby to its mother is a rich source of stem cells, which can treat diseases like leukemia. They can be collected immediately after birth and stored in freezers by companies such as Cordlife, which operates cord blood banks in Hong Kong, Singapore, Indonesia, India and the Philippines. If a child needs it, doctors can retrieve cord blood for treatment.

"We're seeing an explosion umbilical cord blood banking as a source of biological insurance for parents in Asia and their children," said Andrew Wu, Cordlife's technical and laboratory director.

Scientists around the world are finding new ways to use cord blood stem cells to treat problems such as spinal cord damage, diabetes, cerebral palsy and heart disease.

At Cordlife, parents pay about $4,000 to keep their children's cord blood for 18 years.

"I'm most excited to see the use of stem cell in therapy becoming a standard of care, where clinicians when they look at a disease would ask, 'What's my stem cell option in terms of therapy? How I can look to stem cells to treat this disease or regenerate this organ or to combat this tumor?'" Wu said.

Asia appears striding toward the use of stem cells for treatment.

Dr. Supachai Chaithiraphan, a professor emeritus of Thailand's Mahidol University and director of the cardiac center at Chao Phya hospital in Bangkok, conducted a clinical trial in 2004 that injected stem cells derived from human blood to treat people with end-stage heart disease.

"Eighty percent showed improvement in terms of New York Heart Association classification and also the Canadian [Cardiac Society] angina classification. So we feel that this group of patients can derive benefit from their own stem cell therapy," Supachai said.

In Asia, there are fewer regulations regarding the use of stem cells than in the United States, which encourages research and clinical uses.

In the U.S., the use of stem cells has been controversial because of ethical concerns over the use of embryonic stem cells - derived from early-stage embryos that develop from human eggs fertilized in a laboratory. Under President Bush, the government limited funding to a few batches of stem cell lines. But the Obama administration has since relaxed those rules.

However, cord blood storage appears to be increasing in the U.S. A new national cord blood bank has been established at Duke University in the state of North Carolina.

Some practitioners, such as Wu worry that Asia's lax regulations may lead to inflated expectations and false promises.

"A lot still needs to go into building this understanding and building appropriate regulations around the use of these stem cells so that the industry as a whole can develop within a legal framework that not only benefits the companies but most importantly benefits the clinicians and the patients at the end of the day," Wu said.

Poor medical facilities in some developing Asian nations also hold back use of stem cells.

But Supachai says in time, stem cell therapy will be more accessible.

"I would foresee that in the near future more doctors would come to realize that this cell therapy is really of help to certain number of patients with certain diseases, in particular heart disease," Supachai said.

The World Health Organization estimates that about 20 million people worldwide will die from cardiovascular disease annually by 2015. The number of people in developing nations suffering from heart disease is expected to rise as incomes increase.